Download PDF by Phyllis R. Brown: Advances in Chromatography, Volume 40

By Phyllis R. Brown

ISBN-10: 082470018X

ISBN-13: 9780824700188

"Volume forty provides an authoritative collection of the simplest and most modern examine findings in separation technological know-how. Surveys fresh advancements in excessive performance-liquid (HPLC), reversed-phase liquid (RPLC), countercurrent (CCC), and micellar electrokinetic chromatography (MEKC)."

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Example text

The chemical shifts and the relative intensities of the different peaks are the same on both figures. This indicates that the structural characteristics of the chemically bonded ligands are not affected by the reactions of bonding and of chemical cleavage with aqueous hydrofluoric acid. Figure 2 shows the mass spectra of ODDCS and ODDFS and of the products of the cleavage of the ligands of YMC ODS [11]. In Fig. 2a, the molecular ion peak of ODDCS appears at m/z = 346. The relatively intense peak at m/z =331 results from the loss of a methyl group from the molecular ion.

7, respectively [27]. The contribution of the fourth term on the RHS of Eq. (11) may be negligible. Equation (11) can be rearranged as follows: Page 15 This equation will be used in Section IV, in the discussion of the equilibrium constants of the probe compounds used in the experimental part of this work. IV First Moment Analysis: Results and Discussion We discuss here the influence of the experimental conditions on the equilibrium constants of adsorption obtained for the compounds in our data bank, on the value of the reduction of the total hydrophobic surface area observed upon adsorption of a molecule, and on the isosteric heat of adsorption.

5 and 1315), and the value of ke. These three parameters have a significant influence on the calculation results. The parameter ke was introduced to correct the enthalpy change of formation of a cavity in a solvent for the curvature of the surface of the cavity. Horváth et al. [27] estimated the values of ke for different mobile phase compositions. Their calculations provided almost the same results as the experimental value derived from the retention behavior. The value of ke varies with the mobile phase composition.

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Advances in Chromatography, Volume 40 by Phyllis R. Brown


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