By W. B. Hugo, J. B. Stenlake
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Additional info for An Introduction to Microbiology. Pharmaceutical Monographs
Indirect counts which, using a suitable optical instrument, measure the turbidity or light-scattering properties of a suspension. 4. Biochemical methods which measure the increase of, for example, protein in the suspension, again assuming that this is increasing with increasing bacterial numbers. It should be clearly realised that methods 1, 3 and 4 measure either directly or indirectly the total bacterial population, whether living or dead. The Pattern of Bacterial Multiplication Assuming optimum or near optimum conditions, the pattern of the multiplication of bacteria from an inoculum into a liquid medium, is as follows (Fig.
Oxidation-Reduction Potentials of Bacterial Cultures and Anaerobic Growth It is apparent from the information given above that there is a relationship between anaerobic growth and the level of oxidation or reduction in the environment. The quantitative determination of this level is made by measuring a factor known as the oxidationreduction or redox potential. This may be likened to the pH value which is a measure of the degree of acidity or of alkalinity. A full theoretical treatment of oxidation-reduction potentials will be found in textbooks of physical chemistry.
As far as is known, inorganic phosphorus, usually as phosphates, serves as the phosphorus source in nutrition. Growth factors. Examples are given on page 47. The use of the word 'growth factor' enables a miscellaneous group of chemicals to be classified functionally. Some texts would describe an essential amino acid as a growth factor. In this account it is proposed to use the term 'growth factor' in the restricted sense. Many of the substances given are the vitamins of human nutrition. It is of interest to note that if maximum growth is obtained at levels of 10~4 to 10~5M the growth factor or amino acid is probably being built into the cell, whereas if the level for maximum growth is 10~8 to 10~10M the substance concerned is acting as a catalyst, probably as part of an enzyme system.
An Introduction to Microbiology. Pharmaceutical Monographs by W. B. Hugo, J. B. Stenlake