Get Aptamers in Bioanalysis PDF

By M. Mascini

ISBN-10: 0470148306

ISBN-13: 9780470148303

This is often the 1st ebook to aspect bioanalytical applied sciences and techniques which have been built utilizing aptamers in analytical, scientific, environmental, and nutrients technology purposes. After an creation to aptamers, aptamer objectives, and their normal makes use of, it discusses diverse purposes with specific cognizance to the comparability among aptamer-based biosensors and techniques as opposed to the corresponding immunosensors. Examples of aptamer-based diagnostic ideas comprise whole-cell protein profiling (proteomics) and scientific diagnostics for the excellence of diseased as opposed to fit states. it is a center reference for analytical chemists, electrochemists, pharmaceutical/medicinal chemists, biotechnologists, and others.

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Zaia, J. , Rossi, J. , Engelke, D. R. (1997). Expression of small, therapeutic RNAs in human cell nuclei. Gene Ther 4, 45–54. Gopinath, S. C. (2007). Methods developed for SELEX. Anal Bioanal Chem 387, 171–182. , Binkley, J. , Tuerk, C. (1995). Comprehensive chemical modification interference and nucleotide substitution analysis of an RNA pseudoknot inhibitor to HIV-1 reverse transcriptase. J Mol Biol 247, 60–68. Griffin, L. , Tidmarsh, G. , Bock, L. , Toole, J. , Keung, L. L. K. (1993). In vivo anticoagulant properties of a novel nucleoside-based thrombin inhibitor and demonstration of regional anticoagulation in extracorporeal circuits.

2005). Folded RNA regions are not available for intermolecular pairing with the complementary sequence. Consequently, antisense or small interfering RNA targeted to structured regions shows a limited efficiency (Kurreck, 2006). In vitro selection has been carried out to identify aptamers that recognize the folded state of the target RNA region. The numerous interactions identified in tertiary RNA structures, besides Watson–Crick base pairing, suggest that it should be possible to take advantage of nonpaired nucleic acid bases in loops and bulges to engage intermolecular interactions with an aptamer.

The released oligonucleotides are collected and amplified as usual in the SELEX procedure, thus allowing the identification of aptamers whose binding to their ligand induces a conformational change that in turn leads to disruption of the aptamer–capture oligonucleotide complex. If a quencher is introduced on the capture oligonucleotide and a fluorophore into its complementary region on the aptamer, binding of the analyte to the aptamer will result in increased fluorescence emission. This clever approach has been validated for the direct selection of molecular beacons against oligonucleotides (Rajendran and Ellington, 2003), purine nucleotides (Nutiu and Li, 2005b), and more recently, against the aminoglycoside antibiotic tobramycin (Morse, 2007).

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Aptamers in Bioanalysis by M. Mascini

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