By Gérald Thouand, Robert Marks
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Additional resources for Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3
However, the PPy modiﬁcation of the working electrode resulted in an overall decrease of efﬁciency, which could be attributed to a high background signal. 4 CMOS Detection of Bacterial Bioluminescence As discussed previously, although bacterial bioluminescence can be sensitively collected by different photon detectors, it is still a big challenge to design a compact and ﬁeld-portable bacteria biosensor that includes all requested elements, such as incubation, detection, and signal processing. Despite these constraints, some research groups have designed ﬁeld-deployable bioluminescent whole-cell biosensors based on the advanced CMOS technique [48, 49].
5 h (group A) and after an overnight incubation of E. coli TV1061 at 4 °C (group B). 01 ng/mL) were tested for their toxicity to bacterial cells. Bioluminescent measurements for the two modes were recorded at 25 °C was decreased when compared to the major peak obtained within the fresh mode. Thus, the increase of the OD has led to a decrease of the peak amplitude for the overnight mode. Once the optimization of the bacterial OD value was acquired, the toxic effect of atrazine compound to E. coli TV1061 bacteria was investigated through the proposed “fresh” and “overnight” consecutive tests.
Anal Bioanal Chem 400(4):1071–1082 77. Fernández-Pinas F, Wolk CP (1994) Expression of luxCD-E in Anabaena sp. can replace the use of exogenous aldehyde for in vivo localization of transcription by luxAB. Gene 150 (1):169–174 78. Davidov Y, Rozen R, Smulski DR, Van Dyk TK, Vollmer AC, Elsemore DA, LaRossa RA, Belkin S (2000) Improved bacterial SOS promoter∷lux fusions for genotoxicity detection. Mutat Res Genet Toxicol Environ Mutagenesis 466(1):97–107 79. Stocker J, Balluch D, Gsell M, Harms H, Feliciano J, Daunert S, Malik KA, van der Meer JR (2003) Development of a set of simple bacterial biosensors for quantitative and rapid measurements of arsenite and arsenate in potable water.
Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3 by Gérald Thouand, Robert Marks